Methods: Cembrowski et al 2016 “Hipposeq”
Brain regions: Hippocampus
Techniques: Genetic labeling; viral injections in utero; retrograde bead injections; manual cell-sorting; RNA-seq (pooled neurons) with HiSeq 2500 (illumina).
Age of mice: P26-P35
Data processing for BrainPalmSeq: Expression data were downloaded directly from hipposeq.janelia.org and were not further processed.
Heatmap units: Fragments per kilobase million (FPKM)
References: Cembrowski, M.S., Wang, L., Sugino, K., Shields, B.C., and Spruston, N. (2016a). Hipposeq: A comprehensive RNA-seq database of gene expression in hippocampal principal neurons. ELife 5, e14997.
Cembrowski, M.S., Bachman, J.L., Wang, L., Sugino, K., Shields, B.C., and Spruston, N. (2016b). Spatial Gene-Expression Gradients Underlie Prominent Heterogeneity of CA1 Pyramidal Neurons. Neuron 89, 351–368.