Methods: Rosenberg et al 2018

Cell-types: All

Brain regions: Whole brain and spinal cord.

Techniques: Selection-free cell-sampling of C57BL/6 x DBA/2 mice. Single-nucleus RNAseq using SPLiT-seq. 156,049 nuclei were assigned to 73 cell-type (metacell) clusters (e.g. 18 Clau Pyr).

Age of mice: P2 and P11

Data processing for BrainPalmSeq: Single-cell counts were downloaded from GSE110823, and log normalized by first scaling the expression values provided to a sum of 10,000 per cell before calculating log2(scaled_counts+1). Averages were then performed by brain region, neurotransmitter and taxonomy for each gene. Genes associated with palmitoylation were selected in order to create the heatmaps.

Heatmap units: mean log2(counts per 10,000 + 1)


Rosenberg, A.B., Roco, C.M., Muscat, R.A., Kuchina, A., Sample, P., Yao, Z., Graybuck, L.T., Peeler, D.J., Mukherjee, S., Chen, W., et al. (2018). Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding. Science 360, 176–182.

Website: N/A

Original data availability: GSE110823