Methods: Siletti et al 2023

Cell-types: All

Brain regions: Whole brain

Techniques: Human postmortem tissue acquired from donors with no known history of neuropsychiatric or neurological conditions were considered for inclusion in the study. Single cell RNAseq with 10x Chromium. 3,369,219 from 105 dissections.Cells assigned to 31 superclusters, 461 clusters, and finally 3313 subclusters. Expression per cluster is displayed in BrainPalmSeq heatmap.

Age of donors: Male and female donors 18 to 68 years of age

Data processing for BrainPalmSeq: Single-cell counts were downloaded from cellxgene, and log normalized by first scaling the expression values provided to a sum of 10,000 per cell before calculating log2(scaled_counts+1). Averages were then performed by cell type taxonomy for each gene. Genes associated with palmitoylation were selected in order to create the heatmaps.

Heatmap units: mean log2(counts per 10,000 + 1)

References: Kimberly Siletti et al., Transcriptomic diversity of cell types across the adult human brain. Science382,eadd7046(2023). DOI:10.1126/science.add7046

Website: N/A

Original data availability: